Description
Purity>96% SDS-PAGE. estimated also by HPLC.Endotoxin level is less than 0.1 ng per µg (1EU/µg).FunctionChemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 cDNA is located on Chr.5 in humans, and the mRNA of CCL28 is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83 % identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils. MEC/ CCL28 contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines. Recombinant Human MEC/ CCL28 is a 12.3kDa protein containing 108 amino acid residues